Recently I ran into a problem where browsing to a newly installed web app produced a bunch of javascript errors. Stuff like: "'Type' is not defined" and "'Sys' is not defined".
After debugging it for a while, I found the problem to be that URLScan had been installed on the server (Windows 2000 Server), which was preventing any requests with dots in the folder name.
URL Scan is a tool that MS suggested everyone install a while back that acts to filter out many malicious attacks.
So, with the default settings any request for a file inside the scripts\System.Web.Extensions folder would be denied as a 404 b/c of URL Scan.
To fix this, you need to edit the UrlScan.ini file, located in %WINDIR%\System32\Inetsrv\URLscan. Near the top of the file, change AllowDotInPath from 0 to 1.
The run iisreset to restart IIS and you should be ok!
More info on URLScan is available here:
http://support.microsoft.com/kb/326444
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